TRAINING SCHOOL

Basic Methods to Purify and Investigate the Discrete Functions of Human Neutrophils

03-05 MAY 2023

Local Organizers
Prof Marco A. Cassatella
Department of Medicine in the University of Verona
Verona, Italy

Download the Agenda [pdf]
Download the application details [pdf]

CALL FOR APPLICATIONS

Purpose of the Workshop:

The Training School on “basic methods to purify and investigate the discrete functions of human neutrophils” is organized by the COST Action CA18233 European Network for Innovative Diagnosis and Treatment of Chronic Neutropenias (EuNet-INNOCHRON).

The Training School aims at covering aspects on methods to isolate human neutrophils in high purity and measure and quantify some of their functions including Reactive Oxygen Species (ROS), apoptosis and gene expression. The participating scientists will have the opportunity to expand their knowledge via interactive lectures from experts on aspects related to human neutrophils and discuss with them. They will also observe and familiarize themselves with methods to investigate neutrophil functions, discuss, and get hands-on experience on performing specific protocols.
The host of the Training School is Prof Marco A. Cassatella and his team in the Section of General Pathology, Department of Medicine in the University of Verona, Italy.

To offer a high-quality training course including hands-on experience, a very limited number of participants will be accepted.

Deadline for applications: February 6th, 2023

Application Process:

  1. The applicants must send their application to Prof Marco Cassatella email: This email address is being protected from spambots. You need JavaScript enabled to view it. and our COST Action Chair Prof Helen Papadaki, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., before the deadline February 6th 2023.
  2. The application should consist of:
    • A brief C.V.
    • A motivation letter stating the relevance to their research and the value to participate in the Training Course.
    • A letter of support from a senior Researcher/Clinician (preferably but not necessarily related to the EuNet-INNOCHRON project) from their home Institution where they are affiliated to.
  3. The applicants will be formally notified of the outcome of their application on February 13th at the latest.

Important Notes:

  1. The selected applicants will be sent a formal COST invitation to the email they have provided and instructions for reimbursement rules for the expenses regarding the whole travel.
  2. The selected participants will be reimbursed from the COST Action EuNet-INNOCHRON for travel costs and will get a fixed amount of daily allowance (for their accommodation, 2 meals/day and any public local transport). [please refer to COST rules]
  3. To attend this face-to face Training School, participants are subject to the travel regulations of the Italian National Health Authorities.

 

Agenda 

 

Day 1, May 03, 2023

09:00 - 09:30  Introduction to the course
                       [Prof. Cassatella and Prof. Tamassia]
09:30 - 13:00   Practical part: “High-purity isolation of human neutrophils“
                       [Trainers: Dr. Gasperini and Dr. Donini]
13:00 - 14:00   Lunch Break
14:00 - 14:45   Lesson: “Neutrophil responses to Pattern Recognition Receptor (PRRs) activation”
                       [Prof. Tamassia]
14:45 - 15:45   Practical Part: “Methods to measure and quantify Reactive Oxygen Species”
                       [Trainers: Dr. Donini and Dr. Gardiman]
15:45 - 16:00   Coffee Break
16:00 - 17:00   Practical Part:  “Flow cytometric analysis of resting and activated neutrophil phenotype” 
                       [Trainers: Dr. Calzetti and Dr. Pettinella]

 

Day 2, MAY 04, 2023

09:30 - 13:00   Practical part: “High-purity isolation of human neutrophils (Hands-onpractice by attendees)“
                       [Trainers: Dr. Gasperini and Dr. Donini]
13:00 - 14:00   Lunch Break
14:00 - 14:45   Lesson: “Heterogeneity of neutrophils”
                       [Prof. Scapini]
14:45 - 15:45   Practical Part: “Flow cytometric analysis of neutrophil apoptosis”
                       [Trainers: Dr. Calzetti]
15:45 - 16:00   Coffee Break
16:00 - 17:00   Practical Part:  “RNA extraction and reverse transcription” 
                       [Trainers: Dr. Mariotti and Dr. Gardiman]
17:00 - 17:45   Lesson: “Regulation of neutropenia by non-coding RNAs in Large Granular Lymphocyte Leukemia patients”
                       [Prof. Bazzoni]

 

Day 3, MAY 05, 2023

09:30 - 10:30   Practical part: “Real time quantitative PCR“
                        [Trainers: Dr. Mariotti and Dr. Gardiman]
10:30 - 11:15   Lesson: “Neutropoiesis”
                        [Prof. Tamassia and Dr. Calzetti]
11:15 - 11:30   Coffee Break
11:30 - 12:15   Lesson:  “Bone marrow failure syndromes and congenital neutropenia” 
                        [Trainers: Dr. Bezzerri]
12:15 - 13:00   Concluding remarks

 

Name Affiliation
Prof. Flavia Bazzoni Section of General Pathology, Department of Medicine, University of Verona
Dr.  Valentino Bezzerri  Cystic Fibrosis Center, Azienda Ospedaliero Universitaria Integrata di Verona
Dr. Federica Calzetti  Section of General Pathology, Department of Medicine, University of Verona
Prof. Marco A. Cassatella Section of General Pathology, Department of Medicine, University of Verona
Dr. Marta Donini  Section of General Pathology, Department of Medicine, University of Verona
Dr. Elisa Gardiman  Section of General Pathology, Department of Medicine, University of Verona
Dr. Sara Gasperini  Section of General Pathology, Department of Medicine, University of Verona
Dr. Barbara Mariotti  Section of General Pathology, Department of Medicine, University of Verona
Dr. Francesca Pettinella  Section of General Pathology, Department of Medicine, University of Verona
Prof. Patrizia Scapini Section of General Pathology, Department of Medicine, University of Verona
Prof. Nicola Tamassia Section of General Pathology, Department of Medicine, University of Verona

                                                                                                                           * Trainers and Presenters in alphabetical order